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notch3  (R&D Systems)


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    R&D Systems notch3
    Notch3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+notch3+antibody/Human+Notch-3+Antibody/us12338282-387-13-15
    Average 92 stars, based on 14 article reviews
    notch3 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
    Article Snippet: .. Cells were treated with 10 μ M of GSI IX (DAPT) (Calbiochem, Darmstadt, Germany; Cat#565770) for 24 h. In some cases, TALL-1 cells were treated with: 30 μ M proteasome inhibitor MG132 (Sigma, St Louis, MO, USA; Cat#C2211); 10 μg/ml ribosome inhibitor cycloheximide (Sigma; Cat#C4859) for the times indicated; 10 μg/ml of blocking anti-human Notch3 antibody (R&D Systems, Minneapolis, MN, USA; CatAF1559) for 48 h. Purified Sheep IgG (R&D Systems; Cat#5-001-A) was used as an isotype control. ..

    Purification:

    Article Title: Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
    Article Snippet: .. Cells were treated with 10 μ M of GSI IX (DAPT) (Calbiochem, Darmstadt, Germany; Cat#565770) for 24 h. In some cases, TALL-1 cells were treated with: 30 μ M proteasome inhibitor MG132 (Sigma, St Louis, MO, USA; Cat#C2211); 10 μg/ml ribosome inhibitor cycloheximide (Sigma; Cat#C4859) for the times indicated; 10 μg/ml of blocking anti-human Notch3 antibody (R&D Systems, Minneapolis, MN, USA; CatAF1559) for 48 h. Purified Sheep IgG (R&D Systems; Cat#5-001-A) was used as an isotype control. ..

    Control:

    Article Title: Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
    Article Snippet: .. Cells were treated with 10 μ M of GSI IX (DAPT) (Calbiochem, Darmstadt, Germany; Cat#565770) for 24 h. In some cases, TALL-1 cells were treated with: 30 μ M proteasome inhibitor MG132 (Sigma, St Louis, MO, USA; Cat#C2211); 10 μg/ml ribosome inhibitor cycloheximide (Sigma; Cat#C4859) for the times indicated; 10 μg/ml of blocking anti-human Notch3 antibody (R&D Systems, Minneapolis, MN, USA; CatAF1559) for 48 h. Purified Sheep IgG (R&D Systems; Cat#5-001-A) was used as an isotype control. ..



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    Image Search Results


    AAK1 promotes OV progression via Notch3 pathway in vitro . ( A ) Representative immunohistochemical staining images of AAK1 in normal ovary and OV tissue from The Human Protein Atlas (THPA) database. ( B-C ) Relative expression of AAK1 between IOSE-80 cell lines and five OV line were tested by RT-qPCR ( B ) (n = 3) and western blot ( C ) (n = 3), respectively. ( D ) Immunofluorescence staining showing the subcellular localization of AAK1 protein in A2780 and OVCAR3 cells (Scale bar: 10 µm). ( E-G ) CCK8 assay ( E ), Edu assays ( F, Scale bar: 100 µm), and invasion assays ( G ) revealing the cell viability, proliferation, and invasion of A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). ( H ) Western blotting showing the protein levels of AAK1, Notch3, MMP-2, and MMP-9 in A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Journal of Cancer

    Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer

    doi: 10.7150/jca.88359

    Figure Lengend Snippet: AAK1 promotes OV progression via Notch3 pathway in vitro . ( A ) Representative immunohistochemical staining images of AAK1 in normal ovary and OV tissue from The Human Protein Atlas (THPA) database. ( B-C ) Relative expression of AAK1 between IOSE-80 cell lines and five OV line were tested by RT-qPCR ( B ) (n = 3) and western blot ( C ) (n = 3), respectively. ( D ) Immunofluorescence staining showing the subcellular localization of AAK1 protein in A2780 and OVCAR3 cells (Scale bar: 10 µm). ( E-G ) CCK8 assay ( E ), Edu assays ( F, Scale bar: 100 µm), and invasion assays ( G ) revealing the cell viability, proliferation, and invasion of A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). ( H ) Western blotting showing the protein levels of AAK1, Notch3, MMP-2, and MMP-9 in A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Western blotting assay was executed as previously reported , with rabbit anti-human antibodies for AAK1 (1:2000, PA5408, Abmart), Notch3 (1:1000, PS08936, Abmart), GLS (1:2000, T55719, Abmart), MMP-2 (1:1000, PA1748, Abmart), MMP-9 (1:2000, TA5228, Abmart), and β-actin (1:2000, GB11001, Servicebio).

    Techniques: In Vitro, Immunohistochemical staining, Staining, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, CCK-8 Assay, Stable Transfection, Transfection

    AAK1 promotes glutamine metabolism of OV via Notch pathway in A2780 cells. ( A-E ) The cellular Glu ( A ), α-KG ( B ), ATP ( C ), GSH ( D ), and ROS levels (E, Scale bar: 100 µm) of A2780 cells stably transfected as indicated (n = 3). (F) Western blot indicating the protein levels of AAK1, Notch3, and GLS in A2780 and OVCAR3 cells stably transfected as indicated (n = 3). ( G ) Schematic diagram of AAK1 promots glutamine metabolism and malignant progression through the Notch3 pathway in ovarian cancer. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Journal of Cancer

    Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer

    doi: 10.7150/jca.88359

    Figure Lengend Snippet: AAK1 promotes glutamine metabolism of OV via Notch pathway in A2780 cells. ( A-E ) The cellular Glu ( A ), α-KG ( B ), ATP ( C ), GSH ( D ), and ROS levels (E, Scale bar: 100 µm) of A2780 cells stably transfected as indicated (n = 3). (F) Western blot indicating the protein levels of AAK1, Notch3, and GLS in A2780 and OVCAR3 cells stably transfected as indicated (n = 3). ( G ) Schematic diagram of AAK1 promots glutamine metabolism and malignant progression through the Notch3 pathway in ovarian cancer. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Western blotting assay was executed as previously reported , with rabbit anti-human antibodies for AAK1 (1:2000, PA5408, Abmart), Notch3 (1:1000, PS08936, Abmart), GLS (1:2000, T55719, Abmart), MMP-2 (1:1000, PA1748, Abmart), MMP-9 (1:2000, TA5228, Abmart), and β-actin (1:2000, GB11001, Servicebio).

    Techniques: Stable Transfection, Transfection, Western Blot

    Therapeutic knockdown of AAK1 suppresses OV progression and metastasis in vivo . ( A ) The representative images, ( B ) tumor growth, ( C ) tumor weight, ( D ) the representative IHC images and statistical analysis results of AAK1, Notch3, GLS, MMP-2, MMP-9, and Ki-67 of xenograft tumors established by subcutaneous injection of OVCAR3 cells that were stably transfected as indicated, respectively, respectively (n = 5 per group). Scale bar: 50 μm. ( E ) The lung metastasis images (left panel) and metastatic counts (right panel) of tumor lung metastasis assay formed by tail vein injection of OVCAR3 cells that were stably transfected as indicated, respectively (n = 5 per group). Scale bar: 100 μm. ( G ) K-M curves were employed to display the survival of nude mice with distinct treatments in the tumor lung metastasis assay. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Journal of Cancer

    Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer

    doi: 10.7150/jca.88359

    Figure Lengend Snippet: Therapeutic knockdown of AAK1 suppresses OV progression and metastasis in vivo . ( A ) The representative images, ( B ) tumor growth, ( C ) tumor weight, ( D ) the representative IHC images and statistical analysis results of AAK1, Notch3, GLS, MMP-2, MMP-9, and Ki-67 of xenograft tumors established by subcutaneous injection of OVCAR3 cells that were stably transfected as indicated, respectively, respectively (n = 5 per group). Scale bar: 50 μm. ( E ) The lung metastasis images (left panel) and metastatic counts (right panel) of tumor lung metastasis assay formed by tail vein injection of OVCAR3 cells that were stably transfected as indicated, respectively (n = 5 per group). Scale bar: 100 μm. ( G ) K-M curves were employed to display the survival of nude mice with distinct treatments in the tumor lung metastasis assay. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Western blotting assay was executed as previously reported , with rabbit anti-human antibodies for AAK1 (1:2000, PA5408, Abmart), Notch3 (1:1000, PS08936, Abmart), GLS (1:2000, T55719, Abmart), MMP-2 (1:1000, PA1748, Abmart), MMP-9 (1:2000, TA5228, Abmart), and β-actin (1:2000, GB11001, Servicebio).

    Techniques: Knockdown, In Vivo, Injection, Stable Transfection, Transfection